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Ayers has been the subject of scrutiny by the Fox News Channel throughout President Barack Obama's presidency and during some of his 2008 campaign for the Oval Office.
The preview of the interview shows Kelly confrontational with Ayers on several accounts including the acts of violence for which the Weather Underground had been responsible and Ayers' wife Bernardine Dohrn's statements about convicted killer Charles Manson.
The interview is scheduled to air on "The Kelly File" on Monday night.
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